ykwC [2018-02-12T11:09:54.000Z]

beta-hydroxyacid dehydrogenase

locus: BSU13960

pI: 5.11

mw: 30.56 kDa

protein length: 288 aa

gene length: 864 bp

function: unknown

product: beta-hydroxyacid dehydrogenase

essential: no

ec: 1.1.-.-

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,465,733 → 1,466,599

The protein

Protein family

• 3-hydroxyisobutyrate dehydrogenase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|9F8A63C585227D59C8A5CD4356E3F81EB5897131|YfjR] (32%)

Modification

• phosphorylated on Ser-281 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|PrpC], phosphorylation abolishes the oxidoreductase activity of YkwC ''in vitro'' and ''in vivo'' [Pubmed|24390483]

Structure

• [PDB|3WS7] (from ''Pyrobaculum calidifontis '', 42% identity)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|1010B1028A9A5F234C33474C7BC23C9BD2ABF3C4|ykwC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B333 (ykwC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1332 NBRP B. subtilis, Japan]
• BKE13960 (Δ[gene|1010B1028A9A5F234C33474C7BC23C9BD2ABF3C4|ykwC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGTCATTCCTCCCAGAT, downstream forward: _UP4_TAAAAAAAGATTGCCTGTAC
• BKK13960 (Δ[gene|1010B1028A9A5F234C33474C7BC23C9BD2ABF3C4|ykwC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGTCATTCCTCCCAGAT, downstream forward: _UP4_TAAAAAAAGATTGCCTGTAC

References

• 24390483,15378759